FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Pesticide residue, Eleusine indica, phytoremediation, DDVP, rhizosphere interaction
The present study investigated growth performance of Eleusine indica to abiotic stress occasioned by pesticide pollution with a view to ascertaining its suitability for phytoremediation. Soil was spiked with 2,2-dichlorovinyl dimethyl phosphate (DDVP, 1000EC) in solution to obtain 6 different levels of pollution: 5.0, 3.75, 2.5, 0.5, 0.25, and 0.05 ml pesticide/kg of soil. The control was wetted with ordinary water. These treatments were replicated 3 times in completely randomized block design. The entire set up was left to naturally attenuate for 2 days before equal-lengthen 3-leaf tillers of Eleusine indica, the test plant, were transplanted from a nursery. Results showed that within 24 h, young tillers in the 5 ml/kg-soil treatment wilted entirely. Those in 2.5 – 5.0 ml/kg pesticide-impacted soil (PIS) also showed wilting signs, beginning first with folding of plant leaves. There was no evidence of wilting in plants sown in the 0.05 ml/kg PIS and the control. Total pesticide residual content after 11 weeks was 0.512 mg/kg in the 5 ml/kg-PIS (i.e. 10.24% reduction) that lacked plant presence (note, plants died during the first few days). However, Residual content ranged from 0.012 – 0.073 mg/kg in the treatments with plant presence, thus indicating the importance of plant presence. Remediation efficiency was 13.60 – 29.20% in treatments with surviving plant population. Eleusine indica grass showed great promise as a phytoremediator of pesticide. However, the fact that significant morphological changes occurred in the plant also goes to show the effects of increased concentrations of contaminants on the would-be phytoremediators.
Abdul-Ghany O, Sarmamy I & Khidir SM 2003. Effects of some soil treated pesticides on growth characteristics of faba bean & wheat plants. Int. Journ. Emerging Techn. Comput. & Appl. Sci., 5(1): 7 -20. Anoliefo GO, Ikhajiagbe B, Okonokhua BO, & Diafe FV 2006. Ecotaxonomic distribution of plant species around auto mechanic workshops in Asaba and Benin City: Identification of oil tolerant species. Afr. J. Biotech., 5(19): 1757-1762. Anoliefo GO, Ikhajiagbe B, Okonokhua BO, Edegbai BE, & Obasuyi OC 2008. Metal tolerant species distribution and richness in and aroud metal based industries: possible candidates for phytoremediation. Afr. J. Envt. Sci. Techn., 2 (11): 360-370. ASTMD 2013. Standard Test Method for Determination of Polychlorinated Biphenyls (PCBs) in Waste Materials by Gas Chromatography, ASTM International, West Conshohocken, PA, 1998, www.astm.org.DOI:10.1520/D6160-98. Bradford MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analit. Biochem., 72: 248 – 254. Briceño G, Palma G, & Duran N 2007. Influence of organic amendment on the biodegradation & movement of pesticides. Crit. Rev. Envt. Sci. Techn., 37: 233-271. Chaves MM, Maroco JP, & Pereira JS 2003. Understanding plant responses to drought from genes to the whole plants. Funct. Plant Biol., 30: 239-264. Cheesebrough M 2001. District Laboratory Practice in Tropical Countries, Part 2. Cambridge University Press , Cambridge, p. 355. Desi I & Nagymajtenyi L 1999. Electrophysiological biomarkers of an organophosphorous pesticide, dichlorvos. Toxicological Lett., 107(1 – 3): 55 – 64. Diez MC. 2010. Biological aspects involved in the degradation of organic pollutants J. Soil. Sci. Plant. Nutr.,10 3: 244–267. Green JL 2011. Diagnosing Plant Problems. In: Kentucky Master Gardener Manual. ID-194. Cooperative Extension Service, University of Kentucky College of Agriculture, Lexington, UK, pp. 96 -108. Green JL & Capizzi J 1990. A systematic approach to diagnosing plant damage. Ornamentals Northwest Arch., 13(6): 1 – 24. Gyg SP, Corthals GL, Zhang Y, Rochon Y & Acbersold R 2000. Evaluation of two – dimensional gel electrophoresis – based proteome analysis technology. Proc. Natl. Acad. Sci. USA, 97: 9390 – 9395. Huang L, MabT, Li D, Liang F, Liu R, & Li G 2008. Optimization of nutrient component for diesel oil degradation by Rhodococcus erythropolis. Mar. Pollut. Bull., 56: 1714 - 1718. Ikhajiagbe B & Anoliefo GO 2012. Substrate bioaugumentation of spent engine oil polluted soil. Res. J. Env. Earth Sci., 4(1): 60 – 67. Ikpesu TO & Ariyo A B 2013. Health implication of excessive use and abuse of pesticides by the rural dwellers in developing countries: The need for awareness. Greener J. Environ. Mgt. Pub. Safety, 2(5): 180 - 188. Karthikeyan R, Lawrence CD, Larry EE, Kassim A, Peter AK, Philip LB, Stacy LH & Asil AN 2003. Studies on Responses of Non - Target Plants toPesticides: A Review. Departments of biological and agricultural engineering, Biochemistry, chemical engineering, Agronomy, Kansas state University, Manhattan and Institute of plant physiology, Genetics and Bioengineering, Almaty, Kazakhstan. 55p. Konradsen F, Van der Hoek W, Cole DC, Hutchinson G, Daisley H, Singh S & Eddleston M 2003. Reducing acute poisoning in developing countries: options for restoring the availability of pesticides. Toxicol., 192: 249 – 261. Kubis S, Patel R & Combe J 2004. Functional specialization amongst the Arabidopsis Toc159 family of chloroplast protein import receptors. Plant Cell, 16(8): 2059 - 2077. Lawal BO, Torimiro DO, Banjo AD, & Joda AO 2005. Operational Habits and Health Hazards Associated with Pesticide Usage by Cocoa Farmers in Nigeria, Lesson for Extension Work. Institute of Agricultural Research and Training, Moor Plantation, Ibadan, Nigeria, pp. 234 - 250. Mancini F, Van Bruggen AHC, Jiggins JLS, Ambatipudi AC & Murphy H 2005. Acute pesticide poisoning among female and male cotton growers in India. Int. J. Occup. Envt. Health, 11: 221 - 232. Marschner H 1991. Mechanisms of adaptation of plants to acid soils. Plant & Soil, 134: 1 - 20. Obida MG, Stephen SH, Goni AD & Victor OO 2012. Pesticide residues in Bean samples from Northeastern Nigeria. ARPN J. Sci. Technol., 2(2): 79 - 84. Ogbeibu AE 2005. Biostatistics: A Practical Approach to Research and Data Handling. Mindex Publishing Company Limited, Benin City. 264p. Olawale AK & Akintobi OA 2011. Biodegradation of glyphosphate pesticide by bacteria isolated from Agricultural soil. Report & Opinion, 3(1): 124 - 128. Remor AP, Totti CC, Moreira DA, Dutra GP, Heuser IVD, & Boeira JM 2009. Occupational exposure of farm workers to pesticides: biochemical parameters and evaluation of genotoxicity. Envt. Int., 35: 273 - 278. Roche J, Hewezi T, Bouniols A, & Gentzbittel L 2009. Real-time PCR monitoring of signal transduction related genes involved in water stress tolerance mechanism of sunflower. Plant Physiol. Biochem., 47: 139-145. Sabba RNS 1995. Soil Microbiology: Soil Micro-organism and Plant Growth. Oxford Publisher, New Delhi. 509p. Seki M, Umezawa T, Urano K & Shinozaki K 2007. Regulatory metabolic networks in drought stress responses. Cur. Opp. Plt. Biol., 10: 296 - 302. Soares W, Almeida RMVR & Moro S 2003. Rural work and risk factors associated with pesticide use in Minas Gerais, Brazil. Cad. Saude Publica., 19: 1117 - 1127. Steve HD & Jack KC 2004. Pests of Landscaped Trees and Shrubs : An Integrated Pest Management Guide. Regents of the University of California Division of Agriculture and Natural Resources, p. 284. Tashkent P 1998. Part 1. Conditions and Provisions for Developing a National Strategyfor Biodiversity Conservation. Biodiversity conservation National Strategy and Action Plan of Republic of Uzbekistan. Prepared by the National Biodiversity Strategy Project Steering Committee with the Financial assistance of the Global Environmental Facility (GEF) and Technical Assistance of United Nations Development Programme (UNDP). Thompson K, Hodgson JG, Grime JP & Burke MJW 2001. Plant traits and temporal scale : evidence from a 5 year invasion experiment using native species. J. Ecol., 89: 1054 - 1060. Udoh AJ 1998. Safety research study in Nigerian. Farm households hazards. J. Pest. Trust, 40: 5 - 8. U. S. Centres for Disease Control Agency for Toxic Substances and Disease Registry (ATSDR) 1997. Toxicological profile for Dichlorvos. http://www.atsdr.cdc.Gov/toxprofiles/tp888.pdf. U.S. Environmental protection Agency 2006. Interim reregistration eligibility decision for dichlorvos (DDVP). http://www.epa.gov/oppsrrdi/REDS/ddvp-ired.pdf. Yoshitomi KJ & Shann JR 2001. Corn (Zea mays L. ) root exudates and their impact on 14C – pyrene mineralization. Soil Biol. Biochem., 33: 1769 - 1776. Zhao Q, Zhang H, Wang T, Chan S & Dai S 2013. Proteomics – based investigation of salt – responsive mechanisms in plant roots. J. Proteomics, 82: 230 - 253.
